Welcome, Guest. Please login or register.
Did you miss your activation email?
January 17, 2018, 05:01:24 PM

Login with username, password and session length
Search:     Advanced search
1793453 Posts in 224059 Topics by 49540 Members
Latest Member: ghipes
* Home Help Search Calendar Login Register
Taxidermy.Net Forum  |  Beginners, Training & Tutorials  |  Tutorials  |  Topic: Diaphonization « previous next »
Pages: [1] Print
Author Topic: Diaphonization  (Read 3270 times)
New Member
Location: Texas
Posts: 11


WWW Email
« on: June 11, 2015, 07:05:51 PM »

Hello anyone who reads my post,

My name is Kelly and I have been filming a YouTube series entitled "Dyeing The Dead!" (http://www.youtube.com/TacoKellz) I am filming my journey through diaphonization!

As of today, I have completed all of the steps following up to the Trypsin Digestion. It's on this step that I am stuck on.
The protocol I am using is entitled "Give New Life to Old Specimens Through Clearing & Staining" by Bob Weck and Pam Miljak.

The protocol says:
"The specimen is transfered to a trypsin solution for one to several days or until the body is limp and the skeleton becomes visible. Change the solution if the process takes more than three days or if the solution becomes blue. Larger specimens may take more than a week."

I prepared 30 mL of a saturated Sodium Borate solution, added 70 ml of distilled water, then added 1.0 g of Trypsin, and mixed it as well as I could.
My solution became a "cloudy yellow" and developed some small floating pieces. I placed my specimens (mice) into the solution and left over night. When I checked on the solution the next day, it had turned quite blue-ish. My mice had become quite limp but the Alcian blue cartilage stain however was still present on the tissues, tail, and other extremities (feet/hands).
Since my solution had turned "blue," I prepared a fresh Trypsin Solution which I put my mice in and then have been letting them sit for another day.

Can anyone PLEASE HELP ME and possibly give me some advice on what the mice should look like and if I am in fact doing this step correctly? PLEASE!!
This is my first time doing this so I'm not really sure what I'm supposed to be looking for.

Below I have posted some pictures of what my specimens look like as of the second day in a fresh trypsin bath. As you can see, the tail and tissues still appear to be blue. According to the paper, I was under the impression that the Alcian Blue Cartilage Stain was supposed to be removed from the noncartilageous tissue. Looking at the pictures, I still see a lot of blue. The mice also don't seem transparent just yet.

I'd really appreciate it if anyone could help me out. That would be fantastic!
New Member
Posts: 1

« Reply #1 on: July 03, 2017, 05:05:04 PM »

I know this is old, but I've watched your YouTube videos and was curious if you got this figured out...
New Member
Posts: 1

« Reply #2 on: October 18, 2017, 06:21:57 AM »

Wow... really interesting. Thanx for that guide

Thanx for that site because here I have found really cheap insurance quotes. Very useful stuff, guys
Pages: [1] Print 
Taxidermy.Net Forum  |  Beginners, Training & Tutorials  |  Tutorials  |  Topic: Diaphonization « previous next »
Jump to:  

Powered by MySQL Powered by PHP
Contents © 2006-2017 Taxidermy.Net, LLC. All rights reserved. © 2017 Carbon Media Group Outdoors. Privacy Policy.
Powered by SMF 2.0.9 | SMF © 2011, Simple Machines
| TOS | Privacy
Valid XHTML 1.0! Valid CSS!
Page created in 0.06 seconds with 33 queries.